antirat cd43 (1:100, w3/13, bio‐rad)  (Bio-Rad)

Journal: The Journal of Pathology

Article Title: Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathways

doi: 10.1002/path.5890

Figure Lengend Snippet: P2RX7 KO rats are not protected from experimental autoimmune glomerulonephritis. (A) Proteinuria during the 28‐day course of EAG. (B) Urine protein: creatinine ratio (uPCR) at day 28 after induction of EAG. (C) Haematuria during the 28‐day course of EAG. (D) Quantification of glomerular crescents. (E) Quantification of glomerular CD68+ cell infiltration. (F) IL‐1β production from nephritic glomeruli at day 28 after disease induction cultured ex vivo for 48 h. IL‐1β production expressed per 1,000 glomeruli. (G) Titres of circulating anti‐ α3(IV)NC1 antibody levels throughout the 28‐day course of EAG. (H) Quantification of direct immunofluorescence for deposited anti‐GBM antibodies at day 28 after induction of EAG using antirat IgG FITC. (I) Representative photomicrographs showing severe cellular crescents with H&E stain. (J) Photomicrographs showing representative immunoperoxidase staining for CD68+ cells. (K) Representative photomicrographs of direct immunofluorescence for deposited anti‐GBM antibodies. See also supplementary material, Figure . N = 6/group from one of two independent experiments. All rats are included at each timepoint for serial measurements of urinary abnormalities. Data are shown as median with IQR and statistical analysis performed using compared Kruskal–Wallis test with Dunn's post hoc correction. Original magnification of images ×400.

Article Snippet: Immunohistochemistry was performed on formalin‐fixed paraffin‐embedded tissues using the primary antibodies antirat CD68 (1:500, ED1, Bio‐Rad, Hercules, CA, USA), antirat CD43 (1:100, W3/13, Bio‐Rad), or antirat MHC II (1:100, OX6, Bio‐Rad) and the number of positive cells per glomerular cross‐section quantified using automated image analysis.

Techniques: Cell Culture, Ex Vivo, Immunofluorescence, Staining, Immunoperoxidase Staining

Journal: Scientific Reports

Article Title: Streptococcus mutans induces IgA nephropathy-like glomerulonephritis in rats with severe dental caries

doi: 10.1038/s41598-021-85196-4

Figure Lengend Snippet: Comparison of IgA-positive and IgA-negative samples from rats inoculated with Cnm-positive S. mutans . ( a ) Mesangial proliferation scores, ( b ) CD43-positive areas, and ( c ) CD68-positive areas were compared in IgA-positive and -negative samples from the Cnm (+) S. mutans group. Statistical significance was determined using the Student t-test. * P < 0.05, *** P < 0.001.

Article Snippet: The primary antibodies were mouse anti-rat CD43 (W3/13) (BD Biosciences) and anti-CD68 (ED1) (BD Biosciences).

Techniques:

Journal: Frontiers in Immunology

Article Title: Dim Light at Night Impairs Daily Variation of Circulating Immune Cells and Renal Immune Homeostasis

doi: 10.3389/fimmu.2020.614960

Figure Lengend Snippet: Dim light at night (dLAN) disturbs the daily variation and alters the number of circulating immune cells. (A–C) Flow cytometric analysis of peripheral white blood cells (WBCs) collected at ZT9 and ZT21 from rats exposed to either the control light–dark (LD) regime (CTRL) or dLAN (~2 lx) for 2 (upper rows) and 5 (lower rows) weeks. Data represent the mean ± SEM with n = 6–9 per group. (B) Numbers of CD4 + helper (Th) and CD8 + cytotoxic (Tc) T cells and the CD4/CD8 ratio. (C) Numbers of classical (CD43 lo HIS48 hi ) and non-classical (CD43 lo HIS48 hi ) monocytes. Significant differences were evaluated by two-way repeated ANOVA with the Tukey post hoc test for multiple comparisons. Only significant main effects (ZT and dLAN) or interactions (ZT×dLAN) are shown. Dotted lines indicate significant differences between individual groups if an interaction was significant. # P < 0.1, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following fluorochrome-conjugated monoclonal antibodies were used: PE-Cy7 anti-rat CD45 (clone OX-1; Sony Biotechnology, USA; 1611065), FITC anti-rat CD3 (clone G4.18; Thermo Fisher Scientific, USA; 11-0030), APC anti-rat CD4 (clone OX-35; Thermo Fisher Scientific; 17-0040), PE anti-rat CD8a (clone OX-8; Thermo Fisher Scientific; 12-0084), PE anti-rat CD45RA (clone OX-33; Thermo Fisher Scientific; MR6404), APC anti-rat CD161a (clone 10/78; Thermo Fisher Scientific; MR6805), FITC anti-rat granulocytes (clone HIS48; Thermo Fisher Scientific; 11-0570), and PE anti-rat CD43 (clone W3/13; Sony Biotechnology; 1614060).

Techniques: Control

Journal: Physiological Genomics

Article Title: Mycophenolate mofetil prevents cerebrovascular injury in stroke-prone spontaneously hypertensive rats

doi: 10.1152/physiolgenomics.00110.2016

Figure Lengend Snippet: Renal immune cell infiltration. Immunohistochemistry was used to detect CD43+ lymphocytes (top) and CD68+ macrophages (bottom) infiltration in the kidneys collected from vehicle-treated (−MMF) or MMF-treated (+MMF) rats at the end of the treatment protocol or at the time of euthanasia. Representative images are shown above. Scale bar = 50 μm for CD43 and 20 μm for CD68 images. A large number of infiltrating CD43+ and CD68+ cells (indicated by arrows) were detected in the kidneys of vehicle-treated SHR-A3 rats. Immune cell infiltration was markedly reduced by immunosuppression with MMF for 8 wk; n = 8–9. *P < 0.05 vs. vehicle-treated (−MMF) rats.

Article Snippet: CD68 (clone ED1, ABD Serotec) was used as a marker of activated microglia/macrophages, and CD43 (clone W3/13, ThermoFisher Scientific) was used to identify lymphocytes.

Techniques: Immunohistochemistry

Journal: Frontiers in Neurology

Article Title: Lesion Size Is Exacerbated in Hypoxic Rats Whereas Hypoxia-Inducible Factor-1 Alpha and Vascular Endothelial Growth Factor Increase in Injured Normoxic Rats: A Prospective Cohort Study of Secondary Hypoxia in Focal Traumatic Brain Injury

doi: 10.3389/fneur.2016.00023

Figure Lengend Snippet: Primary and secondary antibodies .

Article Snippet: Leukosialin (CD43, W3/13) , Serotec , MCA54 , 1:250 , Cy5, anti-mouse, 715-175-150, Jackson Laboratories, 1:200 , 0.28 , 36.

Techniques:

Journal: Frontiers in Neurology

Article Title: Lesion Size Is Exacerbated in Hypoxic Rats Whereas Hypoxia-Inducible Factor-1 Alpha and Vascular Endothelial Growth Factor Increase in Injured Normoxic Rats: A Prospective Cohort Study of Secondary Hypoxia in Focal Traumatic Brain Injury

doi: 10.3389/fneur.2016.00023

Figure Lengend Snippet: Immunofluorescence of the analyzed proteins . The image illustrates representative immunofluorescence to the following proteins: (A) HIF-1α (HIF) (day 1), (B) VEGF (day 1), (C) C5b-9 (day 1), (D) IgG (day 1), (E) Activated Caspase 3 (day 1), (F) Granulocytes (CD43, day 1), (G) Proliferating microglia (CD34, day 7), and (H) Macrophages (ED1, day 7) (all red, respectively) at 10× amplification from normoxic animals. NeuN was used to label neurons (green). DAPI was used as counterstaining for all cells (blue). All pictures are taken from the peri-lesional area; the cavity is located to the right of each picture. Scale bar = 100 μm.

Article Snippet: Leukosialin (CD43, W3/13) , Serotec , MCA54 , 1:250 , Cy5, anti-mouse, 715-175-150, Jackson Laboratories, 1:200 , 0.28 , 36.

Techniques: Immunofluorescence, Amplification

Journal: Frontiers in Neurology

Article Title: Lesion Size Is Exacerbated in Hypoxic Rats Whereas Hypoxia-Inducible Factor-1 Alpha and Vascular Endothelial Growth Factor Increase in Injured Normoxic Rats: A Prospective Cohort Study of Secondary Hypoxia in Focal Traumatic Brain Injury

doi: 10.3389/fneur.2016.00023

Figure Lengend Snippet: Analyzes of lesion size, edema and immunofluorescence . Lesion size, edema, and immunofluorescence data analysis in hypoxic and normoxic rats: (A) , Lesion size using DAPI staining, (B) Lesion size using NeuN staining, (C) Cytotoxic edema on MRI, (D) Vasogenic edema on MRI, (E) HIF-1α (HIF), (F) C5b-9, (G) Activated caspase-3, (H) CD34, (I) VEGF, (J) IgG, (K) CD43, and (L) ED-1 immunofluorescence intensity. Y -axis represent relative intensity. X -axis represents days after injury (log). Blue dots indicate normoxic while red dots represent hypoxic rats; dotted lines delineate regression lines. The gray area surrounding each line is the 95% confidence interval. Lesion area using DAPI and NeuN, HIF, C5b-9, Caspase-3, CD34, IgG, and ED1 changed significantly over time. Lesion Size, NeuN ( p = 0.02), HIF ( p = 0.01), and VEGF ( p = 0.03) are significantly different between normoxic/hypoxic groups.

Article Snippet: Leukosialin (CD43, W3/13) , Serotec , MCA54 , 1:250 , Cy5, anti-mouse, 715-175-150, Jackson Laboratories, 1:200 , 0.28 , 36.

Techniques: Immunofluorescence, Staining